Journal: Stem cells and development

Article Title: CD24-positive cells from normal adult mouse liver are hepatocyte progenitor cells.

doi: 10.1089/scd.2010.0352

Figure Lengend Snippet: FIG. 3. CD24 expression in normal, untreated, and DDC-treated liver. (A–F) Colocalization of CD24 and CK19 expression in wild-type untreated and DDC-treated livers. CD24 expression on bile duct epithelium was weak in untreated liver compared with red blood cells (A). Note that red blood cells in the vein (A) are strongly stained with CD24, but are CK19 negative in the merged view. To eliminate the high background signal from the red blood cells, the microscopic field was limited to the bile duct area outlined in yellow. The panel below each frame (A–C) shows staining of cells in ductular regions. CD24 (A, D, red) colocalized with CK19 (B, E, green) in the periportal zone in both untreated and DDC-treated liver (C, F). CD24 is induced in DDC-treated ductal cells (D), which correlated with the expansion of expression of CK19 (E). The magnification is 400·. (G–N) Immunohistochemical staining of CD24 and A6 antibodies on adjacent sections of normal wildtype and DDC-treated liver. In normal, untreated, wildtype liver, CD24 was expressed on red blood cells in the vein (labeled by lines) and ductal cells (G, H, green). In the same location of adjacent slides, A6 expression showed ductular staining similar to that of CD24 (I, J, green). In DDC-treated liver, the ductal expansion of CD24 expression (K, L, green) was similar to that of A6 (M, N, green). DNA is stained with DAPI blue. The magnification is 200·. FITC, fluorescein isothiocyanate. Color images available online at www.liebertonline.com/scd

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse anti-rat IgG2a secondary antibody (Serotec; MCA278F, 1:100) was used to detect CK19 specifically.

Techniques: Expressing, Staining, Immunohistochemical staining, Labeling

Journal: Human vaccines & immunotherapeutics

Article Title: The rLVS Δ capB / iglABC vaccine provides potent protection in Fischer rats against inhalational tularemia caused by various virulent Francisella tularensis strains.

doi: 10.1080/21645515.2023.2277083

Figure Lengend Snippet: Figure 5. Total serum IgG antibody in rats pre- and post-challenge (survivors) with Type A F. tularensis FRAN244/Schu S4 strain. Animals were immunized and challenged as described in the legend to Figure 2. Serum IgG antibody and subclasses specific to irradiated FRAN244 (Type A antigen) were assayed. a. Total serum antibody and subclasses in immunized rats prior to challenge with aerosolized F. tularensis Type A FRAN244/Schu S4 strain. For IgG, IgG2a, IgG2b, and IgG2c, all vaccinated groups had significantly greater titers than the PBS group (p<0.01-p<0.001). For IgG1, all vaccinated groups except LVS, rLVS 107 x1 rLVS 107 x2 groups had significantly greater titers than the PBS group (p <0.05 - p <0.001). Values represent median with interquartile range (Q1 and Q3 for the whiskers) of serum antibody for n = 7 or 8 per group. b. Total serum antibody and subclasses in immunized rats surviving challenge with aerosolized F. tularensis Type A strains. Values represent median with interquartile range (Q1 and Q3 for the whiskers) of serum antibody. Notes: in Fischer rats, IgG2b/2c indicates a Th1 type response and IgG1/2a indicates a Th2 type response. Values that are significantly different between two groups are marked with asterisk(s) over an open horizontal line crossing above the two groups. * p <0 .05; ** p < 0.01; and *** p < 0.001. Additional descriptive statistical values are included in Supplemental Tables S2–5.

Article Snippet: After the plates were washed as previously mentioned, diluted goat anti-rat-IgG, -IgG1, -IgG2a, -IgG2b, or -IgG2c horseradish peroxidase conjugate (1:5,000; Southern Biotechnology Associates, Inc.) was added to the wells and the plates were incubated for 30 min at 37°C.

Techniques: Irradiation

Journal: Human vaccines & immunotherapeutics

Article Title: The rLVS Δ capB / iglABC vaccine provides potent protection in Fischer rats against inhalational tularemia caused by various virulent Francisella tularensis strains.

doi: 10.1080/21645515.2023.2277083

Figure Lengend Snippet: Figure 6. Total serum IgG antibody in rats immunized with rLVS vaccines prior to and post challenge with Type A F. tularensis FRAN254 or Type B FRAN255 strain. Animals were immunized and challenged as described in the legend to Figure 3. Sera were collected in immunized rats prior to and after challenge with Type A or Type B strains and assayed for total IgG antibody specific to irradiated FRAN244 (Type A antigen) and FRAN255 (Type B antigen). (a). Prior to challenge; All vaccinated groups in Panel a had significantly greater titers than the PBS group (p <0.01-p <0.0001). (b). Post challenge (survivors only). Values represent median with interquartile range (Q1 and Q3 for the whiskers) of serum antibody titers for n = 6–8/group. * p < 0.05; ** p <0 .01; and *** p < 0.001. Additional descriptive statistical values are included in Supplemental Tables S6,7.

Article Snippet: After the plates were washed as previously mentioned, diluted goat anti-rat-IgG, -IgG1, -IgG2a, -IgG2b, or -IgG2c horseradish peroxidase conjugate (1:5,000; Southern Biotechnology Associates, Inc.) was added to the wells and the plates were incubated for 30 min at 37°C.

Techniques: Vaccines, Irradiation

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 1. PDL1 expression in cancer and noncancer cell lines. A, relative expression of PDL1 from total RNA isolated from cell lines. B, representative PDL1 protein expression levels in corresponding high- and low- expressing cell lines by IHC. C, representative PDL1 protein expression levels in corresponding high- and low- expressing cell lines by flow cytometry.

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Expressing, Isolation, Cytometry

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 2. Relative expression of PDL1 in 38 osteosarcoma specimens. PDL1 expression was evaluated from total RNA by quantitative real-time RT-PCR and showed that the expression levels range over 4 log (5,000-fold).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Expressing, Quantitative RT-PCR

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 3. Overall survival of 37 patients with osteosarcoma in relation to PDL1 gene expression. The median overall survival for PDL1-low patients was 89 months compared with 28 months for PDL1-high patients, which showed a trend but was not statistically significant (P ¼ 0.0544).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Gene Expression

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 4. Correlation between PDL1 gene expression and TILs by IHC. A, representative TILs in osteosarcoma tissues (400); score 0, no TILs; 1, rare/few TILs; 2, brisk/prominent TILs. B, significant positive correlation was shown between PDL1 gene expression and TILs in patients with osteosarcoma (P ¼ 0.0117).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Gene Expression

Journal: Cancer Immunology Research

Article Title: Programmed Cell Death Ligand 1 Expression in Osteosarcoma

doi: 10.1158/2326-6066.cir-13-0224

Figure Lengend Snippet: Figure 5. Characterization of the origins of metastases. A, PDL1 expression is significantly higher in metastatic osteosarcoma tumors that originate from lung than from other locations (P ¼ 0.0024). B, TILs also exhibit a positive correlation with pulmonary osteosarcoma metastasis compared with nonpulmonary metastasis (P ¼ 0.0443).

Article Snippet: Unfortunately, the ProSci rabbit polyclonal failed to detect PDL1 on the positive control cell line SKOV3, and the XW mouse mAb exhibited unspecific binding on the negative control cell line MCF7 (Supplementary Fig. S1).

Techniques: Expressing